Moreover, as JMJD4 is responsible for the demethylation of RIG-I at K18 and K146, we confirmed that HFD-induced steatosis and STAM hepatocarcinogenesis were both promoted in Jmjd4hep−/− mice, which were similar to those in RIG-I K18M+K146M mutant mice (Fig. 6g, h, Additional file 2: Fig. S6i, j). This evidence concerns the gene RIGI and steatosis.