In addition, co-staining of KC tumor sections with M2 markers CD163 and F4/80 revealed a decrease in double positive cells (M2-like macrophages) in ferrichrome-treated tumors compared to vehicle-treated tumors as assessed with dual immunofluorescence analysis, an effect that was later confirmed in other experiments (IF) (Fig. 2d). This evidence concerns the gene CALCA and neoplasm.