CD274 and neoplasm: Therefore, to investigate the role of ferrichrome on macrophage gene expression of immune checkpoints PD-1, its ligand PD-L1, and PI3Kγ, as well as colony-stimulating factor receptor 1 (CSF-1R) which contributes to TAMs recruitment to the tumor site48, we evaluated the expression of these genes via qPCR analysis after treatment of macrophages with ferrichrome or vehicle for 24 h.