The main cluster populated by the Oasl1−/−Apoe−/− mouse group (EC Sub-cluster 4) showed increased expression of genes associated with pulmonary hypertension and atherosclerosis, including Selp, Edn1, Ctla2a, Ccl2, Mmrn1, Plvap, and Lgmn (Fig. 4h, Supplementary Fig. 7b). The gene discussed is EDN1; the disease is pulmonary hypertension.