In our study, GO analysis of leukaemia cells revealed that PI3K/AKT pathway was activated in leukaemia cells co-cultured with T-ALL derived MSCs (Fig. S2B) and the protein expression level of p-PI3K, p-AKT, p-mTOR in leukaemia cells were also increased after co-culture with T-ALL derived MSCs. The gene discussed is AKT1; the disease is leukemia.