In line with this notion, SphK2 deficiency differentially regulated ceramide and SM levels in HFHSD-fed mouse livers and FFA-treated Huh7 hepatic cells, leading to a profound decrease in SM/ceramide ratio (Fig. 4F, I), which was associated with its anti-HCC phenotype (Fig. 1). This evidence concerns the gene SPHK2 and hepatocellular carcinoma.