In this context, the correlation between the proportion of CIBERSORTx defined CD4 + Tfh cells and B cell IGS with ICI response are also consistent with TLS playing an important role in ICI response in the UC-GENOME cohort as multiple reports in breast cancer have shown that CD4 + Tfh cells lie in close proximity to germinal center B cells and are important regulators of antigen specific B cell responses19,32. The gene discussed is CD4; the disease is breast cancer.