After adjustment for BMI using analysis of co-variance (ANCOVA), the significant differences in waist circumference, fasting serum insulin, and HOMA2-%S between NGT and T2DM subjects were disappeared, whereas the increase in FBG, HOMA-IR, and HbA1c and the decrease in QUICKI in T2DM subjects compared to NGT subjects remained significant (P <0.05). This evidence concerns the gene INS and type 2 diabetes mellitus.