To further characterise HIV specific CD8+ T-cells in tonsils, we applied an optimized SMART-seq2 protocol to perform scRNAseq on single-cell tetramer sorted HIV- and CMV-specific CD8+ T-cells as well as bulk CD8+ T-cells (non-specific CD8+ Tet-), from tonsils of participants with chronic untreated HIV infection (Table S2; Figure 5A). Here, CD8A is linked to HIV infectious disease.