Consistent with the RNA-seq data, the mRNA levels of COL5A2 and HOXB4 were lower and UNC5B mRNA levels were higher in RSL3-treated cells than in control cells (Figures 12A–F), indicating the probable roles of the three FAGs in the ferroptotic processes of osteosarcoma cells. This evidence concerns the gene HOXB4 and osteosarcoma.