PCSK9 and familial hypercholesterolemia: By examining the three novel variants of PCSK9 two of them (Gly59Arg, Ala68Asp) occurring in the pro-domain (Fig. 3A-B) in the same individual, a 25- year-old male classified as ‘possible’ FH, with LDL-C levels of 6 mmol/L and self-reported hypercholesterolemia.