NOS1 and ischemia: In another series, spreading ischemia was induced by topical application of artificial cerebrospinal fluid (aCSF) on the brain containing the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine (L-NNA) and increased K+ concentration ([K+]aCSF) as reported previously.5,12,56,64 Spreading ischemia was compared between four groups: SHRsp and WKY on Japanese diet and on regular diet.