NOS1 and ischemia: With regard to the induction of spreading ischemia with a NOS inhibitor and increased [K+]aCSF in rats, it is relevant, that prolonged exposure of tissue to elevated [K+]aCSF results in an ex vivo detectable decrease in the α2/α3NaKA fraction and that elevated [K+]aCSF can be replaced in the pharmacological protocol for induction of spreading ischemia by an ouabain concentration that inhibits α2NaKA but not α1NaKA.56