DNMT3A and acute myeloid leukemia: To determine whether sgRNAs targeting different DNMT1 functional domains exhibited comparable types of mutations across AML cell lines, we transfected individual sgRNAs into OCI-AML2 (DNMT3A mutant) and THP-1 cells (DNMT3A wild-type), which were very sensitive to DNMT1 mutations, and OCI-M1 cells (DNMT3A wild-type), which were less sensitive to DNMT1 mutations.