ProTiler was run on individual sgRNAs spanning either DNMT1 (Fig. 5a) or DNMT3B (Fig. 5b) in AML cell lines wild-type for DNMT3A. Several CKHS regions were identified within the DNMT1 protein in NOMO-1 and THP-1 cells, spanning the RFTS, BAH1, BAH2, and MTase domains. Here, DNMT3A is linked to acute myeloid leukemia.