Consistently, Western blot analysis showed that overexpression of DUXAP8 significantly decreased γ-H2AX (a marker for DNA damage) levels after irradiation, while the knockdown of DUXAP8 showed the opposite effects (Figure S2), which further indicates that overexpression of DUXAP8 reduces the level of DNA damage upon radiation in BC cells. The gene discussed is DUXAP8; the disease is breast cancer.