After the tumor reached a palpable size (7 days), one set of 4T1_FRG1_KD mice (n = 4) was intraperitoneally administrated with anti-GM-CSF neutralizing monoclonal antibody (mAb) (10 mg/kg body weight), and the other set of 4T1_FRG1_KD mice (n = 4) was injected with control IgG antibody (10 mg/kg body weight) alternative days till day 21. The gene discussed is CSF2; the disease is neoplasm.