Mice of the 4 genotypes — WT, Nek8+/jckeIF2α+/SA (double heterozygotes), Nek8jck/jckeIF2α+/+ (jck), and jckeIF2α+/SA — were generated (Figure 3A), and renal cyst burden was determined by ultrasonography at 3 months of age (Figure 3B). Here, NEK8 is linked to cystic kidney disease.