Finally, the target gene-TF-miRNA feed-forward loop demonstrated that the critical regulatory network modules include has-mir-24-3p, has-mir-124-3p, has-mir-155-5p and has-mir-1-3p, which advances our understanding of the molecular complexity of ESR1 dysfunction induced neuroinflammation as a causative factor of the AD process. Here, ESR1 is linked to Alzheimer disease.