To further confirm whether the inflammasome activation induced by ASFV-ΔH240R infection depends on NLRP3, the levels of IL-1β and caspase 1 in wild-type PAMs or the PAMs with NLRP3 knockdown infected with ASFV-ΔH240R or ASFV-WT were measured. The gene discussed is IL1B; the disease is infection.