In this study, we confirmed that the phosphorylation of IκBα and p65 and the transcription of pro-IL-1β were more highly induced by ASFV-ΔH240R infection than ASFV-WT (Fig. 1 and 6), indicating that NF-κB signaling and inflammasome activation were induced in the ASFV-ΔH240R-infected PAMs. This evidence concerns the gene NFKB1 and infection.