As shown in Fig. 9A and B, the viral genome copies and the viral titers of ASFV-ΔH240R in the inhibitor-treated PAMs were increased by 25.1- and 9.1-fold, respectively, compared with those in the mock-treated PAMs (P < 0.01), but there were no significant differences between the medium- and inhibitor-treated PAMs upon ASFV-WT infection, indicating that inhibition of the NF-κB signaling significantly promotes the replication of ASFV-ΔH240R in PAMs. This evidence concerns the gene NFKB1 and infection.