To determine if the NF-κB signaling pathway is specifically activated by ASFV-ΔH240R infection, BAY11-7082, an inhibitor of the NF-κB signaling pathway, was used to treat PAMs, and the transcription levels of pro-IL-1β and the phosphorylation levels of IκBα and p65 were analyzed at 12 and 24 hpi as described above. This evidence concerns the gene NFKBIA and infection.