(1) Large solid thyroid nodules with the volume more than 25 ml;(2) benign lesions confirmed by US examinations;(3) reaching normal and clear cytologic results in US-guided core-needle biopsy;(4) ordinary serum thyroid hormone degree and normal calcitonin level; and(5) obvious neck symptoms or cosmetic concerns. The gene discussed is CALCA; the disease is thyroid nodule.