To further phenotype mouse AF+MHCII+ macrophages in skin and TC-1 tumor, we then evaluated the differential expression of a selected set of macrophage markers (CD11b, CD64, MHCII, TIM-4, CD206, Ly6C, CD11c) and autofluorescence in live CD45+CD11b+MHCII+CD64+ cells from tumor (T) and perilesional skin (NT skin) using spectral flow cytometry and computational analysis (Figure 3, Supplementary Table 5, Supplementary Figure 6). This evidence concerns the gene ITGAX and neoplasm.