To further phenotype mouse AF+MHCII+ macrophages in skin and TC-1 tumor, we then evaluated the differential expression of a selected set of macrophage markers (CD11b, CD64, MHCII, TIM-4, CD206, Ly6C, CD11c) and autofluorescence in live CD45+CD11b+MHCII+CD64+ cells from tumor (T) and perilesional skin (NT skin) using spectral flow cytometry and computational analysis (Figure 3, Supplementary Table 5, Supplementary Figure 6). Here, TIMD4 is linked to neoplasm.