In this study, we evaluated the therapeutic effects of GXNI through the mouse model with myocardial ischemia/reperfusion injury (MIRI), and revealed the potential mechanisms of GXNI in the treatment of AMI in combination with RNA-seq and network pharmacological analysis, which revealed that CXCR1 plays a key role in it. Here, CXCR1 is linked to myocardial ischemia.