It was worth noting that 2.78% (n = 4, 4/144) of the isolates were positive for cpb2 toxin gene in C. perfringens type A. The Beta-2 toxin was encoded by the cpb2 gene, which is associated with gastrointestinal disease in humans and animals (37) and has been found in many animal species such as neonatal calves (38), chickens (39), and piglets (40). Here, CPB2 is linked to gastrointestinal disease.