In the current work, a liposome nanoparticle (Huang et al., Cancer Metastasis Rev., 2018, 37, 173–187) coated by cationic chitosan (CS) using a controlled layer-by-layer (LbL) process was designed to deliver simultaneous si-KRAS, miRNA-532-3p, and 5-Fluorouracil (5-FU) into CRC cells. This evidence concerns the gene KRAS and colorectal carcinoma.