Moreover, ROC curves showed the except for IL6, IL1β, CXCL8 and CCL2, whose AUC values were 0.559, 0.573, 0.508 and 0.645, the AUC of all other genes were greater than 0.7 (Figure 3B), indicating that IL10, JUN, KRAS, PPARG, STAT3 and TLR4 might be used as biomarkers for distinguishing HF and non-HF samples. Here, TLR4 is linked to hydrops fetalis.