PDE10A and ovarian carcinoma: To investigate a functional role of PDE10A in ovarian cancer cells, we used CRISPR/Cas9 gene editing to generate PDE10A homozygous knockout (KO) clones in two ovarian adenocarcinoma cell lines, OV-90 and SKOV3 SKOV3 PDE10A KO clones were easily confirmed by western-blot given the strong expression of PDE10 in the parental cell (Fig. 2C).