In response to 6-h hypoxia and 6-h ischemia, the methylation rates of all genes were reduced: the Ambra1 methylation rate diminished to 27% (hypoxia) and 24% (ischemia), the Map1lc3b methylation rate decreased to 39% (hypoxia) and 16% (ischemia), the Atg7 methylation rate decreased to 21% (hypoxia) and 15% (ischemia), and the Becn1 methylation rate was reduced to 33% (hypoxia) and 25% (ischemia). This evidence concerns the gene AMBRA1 and ischemia.