BECN1 and ischemia: In the case of ischemia, the silencing of Becn1 expression with specific siRNA also resulted in the loss of the neuroprotective properties of amorfrutin B. However, in Atg7 siRNA-transfected cells, amorfrutin B (1 and 5 μM) still protected cells from ischemia-induced cell death, as evidenced by the decrease in the LDH parameter to 161% compared to the negative control (Fig. 6b).