Our findings demonstrated that monocyte-derived TNF-α production by qRT-PCR and ELISA techniques in thalassemia patients was significantly lower than that in healthy controls (overall between-group p < 0.0001); in response to LPS approximately 1.3–1.7-fold in NTDT, 1.3–2.5-fold in TDT-NS and 1.5–3-fold in TDT-S, which confirmed to earlier result. The gene discussed is TNF; the disease is thalassemia.