CCND1 and prostate intraepithelial neoplasia: IHC analyses further detected positive staining for IGF1R, phosphorylated IGF1R (pIGF1R), and CyclinD1, a downstream target of β-catenin, in atypical cells of PIN lesions in HiMyc mouse tissues (Supplementary Fig. 3h1–4 vs 3i1–4), suggesting activated IGF1R and Wnt signaling pathways.