Using triple-IF approaches, we further identified co-expression of Myc and CK14, as well as pIGF1R, phosphorylated AKT, phosphorylated GSK3β, cytoplasmic and nuclear β-catenin, CyclinD1, or TCF7L2 in atypical basal cells in PIN lesions of HiMyc mice but not those of HiMyc-ARKO mice (p < 0.01; yellow arrows, top panel, Fig. 4e; Supplementary Fig. 3j). The gene discussed is GSK3B; the disease is prostate intraepithelial neoplasia.