However, the number of cBF neurons, a feature of AD that occurs early in the human disease but is not readily observed in the familial mouse models33, was significantly reduced in cMPT-lesioned APP/PS1 mice compared to sham-lesioned APP/PS1 mice (Fig. 5L), as was the density of cholinergic axonal innervation to the cortical regions (Supplementary Fig. 5). This evidence concerns the gene APP and Alzheimer disease.