Immunohistochemistry further confirmed the significantly decreased expression of FOXA1, GATA3 and PPARγ in the SqD versus UC regions of mixed histology bladder tumors (Fig. 3d, Supplementary Table 1, all p < 0.05, paired Wilcoxon test, H-score)40. This evidence concerns the gene GATA3 and urinary bladder neoplasm.