After 2 days, these mice were injected intraperitoneally with a 20 mg/kg dose of monoclonal anti-PD-L1, anti-PD-L2, or anti-PD-L1/PD-L2 blocking antibody, IgG as control antibodies, every 2 days, for a period of 10 days, then tumor tissues were collected and determined (Fig. 9A). This evidence concerns the gene CD274 and neoplasm.