GSDMD and neoplasm: To investigate whether or not GSDMD-mediated pyroptosis mediates the tumor-suppressive effects of Mll4 deletion or combination of Mll4 deletion and anti-PD-1 blockade in vivo, we administered mice with disulfiram to specifically block GSDMD-mediated pyroptosis through inhibiting the pore formation of the cleaved N-terminal GSDMD fragment on plasma membrane72 (Fig. 7k).