Flow cytometry analyses of the TME revealed that GSDMD depletion impairs the infiltration of total and CD8+ T lymphocytes, as well as attenuates the activation and cytotoxic function of intratumoral CD8+ T cells in Mll4−/− melanomas (Fig. 5g–j), indicating transcriptional induction of GSDMD-mediated pyroptosis augments the anti-tumor immune response to suppress Mll4−/− B16 tumor progression in immune-competent mice. Here, KMT2D is linked to melanoma.