Moreover, we found expression of pyroptotic pathway components, including GSDMD, CASP1, and CASP4 is positively correlated with clinical response to anti-PD-1 or anti- PD-L1 blockade treatment and is predictive of clinical prognosis in multiple cohorts of patients with diverse cancers, suggesting the induced expression of Gsdmd and inflammatory caspases confers the increased tumor-suppressive effects of the PD-1 blockade in Mll4−/− murine melanoma model. This evidence concerns the gene KMT2D and melanoma.