GSDMD and neoplasm: Expression of exogenous wild-type but not D276A mutant GSDMD leads to the appearance of giant membrane balloon, extracellular release of LDH and preloaded calcein AM into supernatants, and increased cell death in B16-Ova cells (Fig. 4g, h, and Supplementary Fig. 4g), indicating immune attack by antigen-specific cytotoxic T lymphocytes induces GSDMD cleavage and triggers pyroptotic cell death in tumor cells expressing exogenous GSDMD.