BRCA1 and breast carcinoma: To investigate the effects of MYC overexpression on cGAS/STING signaling in human breast cancer, the TNBC cell line BT-549 was transduced with doxycycline-inducible short hairpin RNAs (shRNA) targeting BRCA1 or BRCA2, with or without constitutive overexpression of MYC (Supplementary Fig. S4F), whereas cGAS−/− cells served as controls.