CCR4 and amyotrophic lateral sclerosis: With manual gating only (eFigure 4, links.lww.com/NXI/A750), naive B cells were less abundant in patients with ALS compared with those in HCs (p < 0.005), A-L had more CD4 T-effector (CD4 T EFF) cells than A-B (p < 0.05) while CCR4-expressing CD4 T EFF cells were more abundant in blood from A-B compared with those from A-L (p < 0.05; eFigure 4, links.lww.com/NXI/A750).