Unsupervised clustering followed by generalized linear modeling, QL F-test, and FDR adjustment revealed a significant difference between patients with ALS and HCs in the abundance of senescent CD4 cells expressing CD57 (CD4 T SEN) (log FC = 3.04, F = 10.01, p = 0.0017, FDR-adjusted p = 0.029; Figure 1C). The gene discussed is CD4; the disease is amyotrophic lateral sclerosis.