Given the sensitive response of knockin mice in LPS-sepsis, and inflammasome activation heavily required for the development of experimental autoimmune encephalomyelitis (EAE) (35, 36), we thus performed the EAE model on GSDMD-NLSL;LysM-Cre mice to further evaluate the inhibitory effect of GSDMD-N fragment on inflammasome activation in vivo. This evidence concerns the gene GSDMD and experimental autoimmune encephalomyelitis.