AIM2 and escherichia coli infection: Next, we examined the role of GSDMD in regulating inflammasome activation by other stimuli, including poly(dA:dT) to activate AIM2-dependent inflammasome, infection with Salmonella typhimurium that activates NLRC4 canonical inflammasome, as well as Escherichia coli infection and LPS electroporation to engage the noncanonical inflammasome pathway.