Next, we examined the role of GSDMD in regulating inflammasome activation by other stimuli, including poly(dA:dT) to activate AIM2-dependent inflammasome, infection with Salmonella typhimurium that activates NLRC4 canonical inflammasome, as well as Escherichia coli infection and LPS electroporation to engage the noncanonical inflammasome pathway. Here, GSDMD is linked to escherichia coli infection.