ERBB2 and neoplasm: To evaluate tumor cell desialylation in vivo, we established orthotopic EMT6-HER2 tumors in BALB/c mice until they reached a size of 400 to 500 mm3, upon which the mice were treated systemically with a single intraperitoneally administered dose of 10 mg/kg of E-301 or trastuzumab (Fig. 2C).