APC and Familial adenomatous polyposis: We also observe increased HSPB1 expression in human colon organoids derived from patients with familial adenomatous polyposis (FAP), carrying heritable mutations in the APC gene predisposing them to the development of adenomas and CRC (Fig EV4A) and confirmed that BVDU treatment decreased clonogenicity of these human FAP (APC−/−) organoids (Fig EV4B and C).