As shown in Fig EV3C–E, overexpression of full‐length Myc‐tagged TOMM34 (Myc‐TOM‐FL) restored the migration and invasion of endogenous TOMM34 knockdown HCC cells, while the Myc‐TOM‐TPR4‐del failed to restore the metastatic potential of cells, indicating that TPR4 region‐mediated TOMM34/ATP5B interaction is responsible for enhanced migratory and invasive capacities of metformin‐adaptive HCC cells. Here, TOMM34 is linked to hepatocellular carcinoma.