Next, we consider a time course of A549 lung carcinoma epithelial cells (n = 3,003 cells) in vitro (Cook & Vanderhyden, 2020) where epithelial–mesenchymal transition (EMT) was induced by a constant dosage of TGFβ, and scRNA‐seq was performed at successive time points (t = 0 days, 8 h, 1 day, 3 days). The gene discussed is TGFB1; the disease is lung carcinoma.