To visually observe the killing effect of B7-H3 CAR-T cells against tumor cells, we repeated the tumor cell killing experiment using U-2 OS tumor cells in a 96-well cell culture plate, and the results clearly showed that the U-2 OS cells in the CAR-T cell group were significantly reduced, and the CAR-T cells clumped in the vicinity of the tumor cells (Fig. 4D). The gene discussed is CD276; the disease is neoplasm.