The results showed that the positive expression rate of B7-H3 protein in the pathological sections of 60 cases of osteosarcoma reached 73.3%, of which 8 cases (13.3%) had High grades and 18 cases (30%) had Medium grades, 18 cases (30%) of Low grades, and 16 cases (26.7%) of Negative expression (Fig. S3, Fig. S4 and Table 1). The gene discussed is CD276; the disease is osteosarcoma.