We further detected the EMT markers in RCC cells transfected with miR-181d-5p mimics or inhibitors, and the results showed that overexpression miR-181d-5p could significantly upregulate the N-cadherin protein level and reduce the E-cadherin protein level, while blocking miR-181d-5p in RCC cells obviously decreased N-cadherin protein expression and enhanced the E-cadherin protein expression (Supplementary Fig. 3C). This evidence concerns the gene CDH1 and renal cell carcinoma.