Althoughboth the targeted nanocarriers with one or three copies of ACUPA ligandssignificantly improved the tumor retention and tissue penetrationin PSMA+ PC3-Pip xenografts, the multivalent targeted nanocarrier[89Zr]PEG-(DFB)1(ACUPA)3 with threeACUPA ligands showed a remarkably higher PC3-Pip/blood ratio and backgroundclearance. The gene discussed is PIP; the disease is neoplasm.