We performed ‘SE-based TF–Gene analysis’ by inputting mouse cardiac enriched TFs (Nkx2-5, Gata4, Mef2c and Mef2d) as TF input and mouse cardiac marker genes (Myh7, Myh6, Tnni3, Tnni1, Gata6, Acta1, Nkx2-5 and Mef2c) as gene input list to exhibit the new usage and potential applications of SEdb 2.0 in mouse model-based research fields, especially in cardiovascular diseases. This evidence concerns the gene TF and cardiovascular disorder.