TGFB1 and persistent truncus arteriosus: Based on heart size, HM/BW, echocardiography, Masson staining, and COL1 and TGF-β expression, we found that after 4 weeks of TAC modeling, significant cardiac fibrosis occurred in the mouse heart, and the degree of cardiac fibrosis was alleviated after removing the flora, which was consistent with previous research results (31).