Data of TCGA-LUSC was applied to discover immune-related genes, three GEO datasets were used for validation, besides IMvigor210 dataset of 348 patients with urothelial carcinoma receiving the treatment of anti-PD-L1 antibody atezolizumab and GSE126045 dataset of 9 patients with LUSC under nivolumab treatment were utilized for identifying the prognostic value of DLX2 on ICI treatment. The gene discussed is CD274; the disease is urothelial carcinoma.