In this study, we used bioinformatics to search for common target genes and pathways between osteoporosis and Cushing syndrome, and found that INS resistance and the resulting high glucose levels in Cushing syndrome can affect the proliferation and differentiation of osteoblasts and apoptosis, while excessive INS causes overexpression of IGF1, which not only affects cartilage synthesis but also aggravates hormonal disorders. The gene discussed is INS; the disease is Cushing syndrome due to macronodular adrenal hyperplasia.