A total of 104 intersecting target genes were found between osteoporosis and Cushing syndrome, accounting for about 8.83%, with core target genes such as INS, TNF, STAT3, IL6, IGF1, etc.; the biological processes involved include regulation of ribonucleic acid polymerase II promoter transcription, involvement in cytokine signaling pathway conduction, etc.; KEGG pathway analysis showed that the 2 diseases involved neurological ligand recipient interaction pathway, JAK-STAT signaling pathway, Rap1 signaling pathway, PI3K-Akt signaling pathway and many other signaling pathways. This evidence concerns the gene INS and Cushing syndrome due to macronodular adrenal hyperplasia.