In the present study we analyzed 6 SNV in ABCB1 gene (3435C > T, rs1045642), (1236G > A, rs1128503), (2677G > T/A, rs2032582); CDA gene (79A > C, rs2072671), (‐451C > T, rs532545); DCK gene (‐201C > A, rs2306744), and the presence or absence of the GSTT1 and GSTM1 null alleles and their correlation with clinical outcomes and toxicity in a cohort of pediatric patients with AML from two pediatric cancer treatment centers in Colombia. This evidence concerns the gene ABCB1 and acute myeloid leukemia.