The α2-3NA modified ID8 DWCTV with low sialic acid residues and high tumor antigenic Gal/GalNAc epitopes in our study induced Siglec Elow/MGLhigh DCs and the increased CD4/CD8 ratio in DLNs and the increased expression of IFNγ and IL-2 in peripheral plasma, indicating that vaccination with DWCTVs could activate both local and systemic immune responses. This evidence concerns the gene CD4 and neoplasm.