Both CCK-8 and plate cloning assays showed that (Fig. 2A, B) the proliferation of EA cells was reduced due to the overexpression of NOD2, while the EDU assay also revealed that (Fig. 2C, D) the lentivirus overexpressing NOD2 could significantly inhibit the proliferation of EA cells. The gene discussed is NOD2; the disease is Esophageal atresia.