The results are shown in Fig. 7A. The injection of NOD2 overexpression lentivirus into the mass successfully increased the expression level of NOD2, increased the protein expression of P-ATG16L1 and LC3II, and reduced the protein content of P62, indicating that the overexpression of NOD2 increased the autophagy level of tumor tissue in vivo. This evidence concerns the gene SQSTM1 and neoplasm.