Targeted regions of T446 RNA sequences included essential genes (major capsid protein (mcp), transcriptional activator motA), a conditionally essential gene (deoxycytidylate hydroxymethylase gp42), a non-essential gene (accessory capsid protein soc), an early-infection gene (motA), a middle-infection gene (gp42), late-infection genes (mcp, soc), encompassing regions early in coding sequences (CDSs) (mcp, soc), middle in CDS (soc) and untranslated regions around the ribosome binding site (RBS) (gp42, motA) (Supplementary Table 1 and Fig. 2). This evidence concerns the gene FREM1 and infection.