EMT induction through EZH2 overexpression in ESCC was demonstrated by changes in cellular morphology from epithelial to mesenchymal which led to metastasis and invasion in EC (Fig. 7), a reduction in E-cadherin protein levels likewise Occludin at mRNA level, and an increase in Vimentin, Fibronectin, N-cadherin and Zeb2 expression levels (Fig. 4). The gene discussed is VIM; the disease is esophageal squamous cell carcinoma.